KOD Dash Ptotocol@(PCR products > 1kb)
| @Examples | |||
| @ | D.W. | 17.0 | @ |
| @ | 10 x PCR buffer | 2.5 | @ |
| @ | dNTPs (2 mM) | 2.5 | @ |
| @ | 5' primer (20 pmol/Κl) | 1.0 | @ |
| @ | 3' primer (20 pmol/Κl) | 1.0 | @ |
| @ | KOD Dash | 0.5 | @ |
| @ | Template | 0.5 | @ |
| @ | |||
| @Cycling (PE 2400) | |||
| 1st | 94 | 30 sec | @ |
| 1-36th | 94 | 30 sec | @ |
| @ | 58 | 10 sec | @ |
| @ | 74 | 40 sec | @(1 kb; 30 sec, 2kb < add 1 kb/30 sec) |
| 36th | 74 | 10 min | @ |
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Last Updated 2005/8/8