Bellier Jean-Pierre
（Molecular Neuroscience Research Center）

Immunohistochemistry and histochemistry are techniques used for the identification and visualization of molecules in cells and tissues. Since their development started by the end of the 19th century, continuous innovations in imaging technology and reagents constantly improves and extends their applications. Nowadays, immunohistochemistry and histochemistry are essential tools in clinical and life science.

This overview explains and describes:
- Basics of histological and staining techniques.
- Principles for optimization and validation of protocols.
- Capabilities of the DeltaVision high-resolution imaging system.
- The basic of 3D-reconstruction and modelling using IMARIS software.