Bellier Jean-Pierre　　
（Molecular Neuroscience Research Center）　

Immunohistochemical and histochemical methods are used for the identification and visualization of molecules in cells and tissues. Since the initial technological development at the end of the 19th century, continuous innovative improvement in imaging technology and reagents constantly extends applications. Nowadays, immunohistochemistry and histochemistry are essential tools in clinical and life science.

This overview explains and describes:
- Basics of histological and staining techniques.
- Principles for optimization and validation of protocols.
- Capabilities of the DeltaVision high-resolution imaging system.
- The basics of 3D-reconstruction and modeling using IMARIS software.