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Introduction to Modern Confocal Microscopy and its Applications
(Lecture (in English))

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Kinji Asahina, Takefumi Yamamoto, Yasuhiro Mori, Tokio Terado (Central Research Laboratory)
Collaborator: Andor Technology, Oxford Instruments


@One of the drivers of novel research breakthroughs in modern Life Sciences is the use of microscopy. Therefore, it is not surprising that researchers use a wide range of microscopy techniques to understand fundamental concepts in Life Sciences and biomedical research. In fact, novel technologies and experimental methodologies are essential to push the boundaries of research.

@In this talk, I will explain the principles of fluorescence microscopy and the differences between widefield and confocal microscopy. In addition, an overview of the differences between multipoint confocal imaging systems (also known as spinning disks) and point scanner confocal imaging systems (also known as laser scanning confocal microscopes) will also be given. Finally, I will introduce Dragonfly, Andor's High-end multipoint confocal. Dragonfly.

@Dragonfly Multimodal Confocal was designed to integrate biological imaging from single cells to a tissue or organism context. Key to this is exceptionally high background rejection in thick samples, a very low noise floor to retain detection of low signal fluorescence as well as high-intensity labelling, and live volume rendering for instant sample exploration.

@Researchers using the Dragonfly confocal platform publish outstanding science in high-profile journals. In this webinar, I will show examples of published data acquired with the Dragonfly multimodal system. In addition, I will present data from different research areas in biomedical science and show different applications/techniques of up-to-date microscopy.


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Last Updated 2022/7/25